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經歷：
2010–迄今 衛生福利部國家中醫藥研究所 研究員
1995– 2000 國立中國醫藥研究所 副研究員
2002–迄今 臺北醫學大學 生藥學系所 兼任副教授
2003–2006 臺灣大學 農藝學研究系所 兼任副教授
學術著作
Mei-Kuang Lu 1, *, Meng-Hsin Lee 1, Chi-Hsein Chao a, Yu-Chi Hsu (2024) Sodium sulfate addition increases the bioresource of biologically active sulfated polysaccharides from Antrodia cinnamomea. International Journal of Biological Macromolecules 257P2, 128699. (IF 8.2, Polymer Science 5/86=5.81%) 1 Equally contribute
Mei-Kuang Lu *, Chi-Hsein Chao, Yu-Chi Hsu (2023) Advanced culture strategy shows varying bioactivities of sulfated polysaccharides of Poria cocos. International Journal of Biological Macromolecules 253, 126669. (IF 8.2, Polymer Science 5/86=5.81%)
Wei-Lun Qiu, Hung-Chih Lo, Mei-Kuang Lu *, Tung-Yi Lin * (2023) Significance of culture period on the physiochemistry and anti-cancer potentials of polysaccharides from mycelia of Ganoderma lucidum. International Journal of Biological Macromolecules 242P4, 125181. (IF 8.2, Polymer Science 5/86=5.81%)
Mei-Kuang Lu, Chi-Hsein Chao, Tsu-Yuan Chang, Ming-Che Cheng, Yu-Chi Hsu, Chia-Chuan Chang * (2023) A branched 2-O sulfated 1,3-/1,4-galactoglucan from Antrodia cinnamomea exhibits moderate antiproliferative and anti-inflammatory activities. International Journal of Biological Macromolecules 241, 124559. (IF 8.2, Polymer Science 5/86=5.81%)
Zhi-Hu Lin1, Mei-Kuang Lu1, Hung-Chih Lo, Chia-Chuan Chang, Ai-Jung Tseng, Chi-Hsein Chao, Tung-Yi Lin* (2023) ZnF3, a sulfated polysaccharide from Antrodia cinnamomea, inhibits lung cancer cells via induction of apoptosis and activation of M1-like macrophage-induced cell death. International Journal of Biological Macromolecules 238, 124144. (IF 8.2, Polymer Science 5/86=5.81%) 1 Equally contribute
Tung-Yi Lin*, Hung-Chih Lo, Wei-Lun Qiu, Chi-Hsein Chao, Mei-Kuang Lu*, Hsien-Yeh Hsu* (2023) Biochemical characterization and anti-cancer activity of tangential flow filtration system assisted purification of fucoglucan from Laminaria japonica. International Journal of Biological Macromolecules 227, 1-9. (IF 8.2, Polymer Science 5/86=5.81%) Co-correspondence
Pu-Yun Kow1, Mei-Kuang Lu1, Meng-Hsin Lee, Wei-Bin Lu, Fi-John Chang* (2023) Developing a hybrid machine learning model for promoting microbe biomass production. Bioresource Technology 369, 128412. (IF 11.4, Agriculture Engineering 1/14=7.1 %) 1 Equally contribute
Mei-Kuang Lu*, Chia-I Jen, Chi-Hsein Chao, Yu-Chi Hsu, Lean-Teik Ng* (2023) SPS, a sulfated galactoglucan of Laetiporus sulphureus, exhibited anti-inflammatory activities. International Journal of Biological Macromolecules 226, 1236-1247. (IF 8.2, Polymer Science 5/86=5.81%)
Mei-Kuang Lu #, *, Chia-Chuan Chang #, Chi-Hsein Chao, Yu-Chi Hsu (2022) Structural changes, and anti-inflammatory, anti-cancer potential of polysaccharides from multiple processing of Rehmannia glutinosa. International Journal of Biological Macromolecules, 206, 621-632. (IF 8.2, Polymer Science 5/86=5.81%) # Equally contribute
Meng-Hsin Lee, Wei-Bin Lu, Mei-Kuang Lu *, Fi-John Chang * (2022) A hybrid of response surface methodology and artificial neural network in optimization of culture conditions of mycelia growth of Antrodia cinnamomea. Biomass and Bioenergy 158, 106349. (IF 6.0, Agricultural Engineering 2/14=14.29%) *Corresponding author
Mei-Kuang Lu*, Chi-Hsein Chao, Yu-Chi Hsu (2021) Effect of carbohydrate-feeding strategy on the production, physiochemical changes, anti-inflammation activities of polysaccharides of Poria cocos International Journal of Biological Macromolecules 192, 435-443. (IF 8.2, Polymer Science 5/86=5.81%)
Mei-Kuang Lu, Chi-Hsein Chao, Yu-Chi Hsu, Chia-Chuan Chang * (2021) Structural sequencing and anti-inflammatory, anti-lung cancer activities of 1,4-α/β-sulfomalonoglucan in Antrodia cinnamomea. International Journal of Biological Macromolecules 170, 307-316. (IF 8.2, Polymer Science 5/86=5.81%)
Tung-Yi Lin # *, Mei-Kuang Lu #, Ai-Jung Tseng, Chi-Hsein Chao (2020) Effects of sterol-type elicitors on biochemical characterization of polysaccharides from Antrodia cinnamomea. International Journal of Biological Macromolecules 162, 1476-1483. (IF 8.2, Polymer Science 5/86=5.81%) # Equally contribute
Meng-Hsin Lee, Chi-Hsein Chao, Yu-Chi Hsu, Mei-Kuang Lu * (2020) Production, characterization, and functions of sulfated polysaccharides from zinc sulfate enriched cultivation of Antrodia cinnamomea. International Journal of Biological Macromolecules 159, 1013-1021. (IF 8.2, Polymer Science 5/86=5.81%)
Tung-Yi Lin §, Mei-Kuang Lu §, and Chia-Chuan Chang * (2020) Structural identification of a fucose-containing 1,3-β-mannoglucan from Poria cocos and its anti-lung cancer CL1-5 cells migration via inhibition of TGFβR-mediated signaling. International Journal of Biological Macromolecules 157, 311-318. (IF 8.2, Polymer Science 5/86=5.81%) § Equally contribute
Mei-Kuang Lu *, Meng-Hsin Lee , Chi-Hsein Chao, Yu-Chi Hsu (2020) Physiochemical changes and mechanisms of anti-inflammation effect of sulfated polysaccharides from ammonium sulfate feeding of Antrodia cinnamomea. International Journal of Biological Macromolecules 148, 715-721. (IF 8.2, Polymer Science 5/86=5.81%)
Chun-Han Su, Mei-Kuang Lu, Ting-Jang Lu, Ming-Nan Lai, Lean-Teik Ng (2020) A (1→6)-branched (1→4)-β-D-glucan from Grifola frondosa inhibits lipopolysaccharide-induced cytokine production in RAW264.7 macrophages by binding to TLR2 rather than Dectin-1 or CR3 receptors. Journal of Natural Products 83, 231-242. (IF 5.1, Plant Science 33/238=13.87 %)
Tung-Yi Lin*, Chia-Chuan Chang, Ai-Jung Tseng, Chi-Hsein Chao, Mei-Kuang Lu* (2019) Sodium thiosulfate enhances production of polysaccharides and anticancer activities of sulfated polysaccharides in Antrodia cinnamomea. Carbohydrate Polymers 216, 204-212. (IF 11.2, Polymer Science 3/86=3.49%)
Tung-Yi Lin*, Ai-Jung Tseng, Wei-Lun Qiu, Chi-Hsein Chao, Mei-Kuang Lu * (2019) A sulfated glucan from Antrodia cinnamomea reduces Slug expression through regulation of TGFβ/AKT/GSK3β axis in lung cancer. Carbohydrate Polymers 210, 175-184. (IF 11.2, Polymer Science 3/86=3.49%)
Mei-Kuang Lu *, Tung-Yi Lin, Chia-Chuan Chang * (2018) Chemical identification of a sulfated glucan from Antrodia cinnamomea and its anti-cancer functions via inhibition of EGFR and mTOR activity. Carbohydrate Polymers 202, 536-544. (IF 11.2, Polymer Science 3/86=3.49%)
Tung-Yi Lin*, Ai-Jung Tseng, Chi-Hsein Chao, Mei-Kuang Lu* (2018) Microelements induce changes in characterization of sulfated polysaccharides from Antrodia cinnamomea. International Journal of Biological Macromolecules 120, 952-958. (IF 8.2, Polymer Science 5/86=5.81%)
Hsien-Yeh Hsu*, Tung-Yi Lin, Chun-Hao Hu, David Ta Fu Shu, Mei-Kuang Lu* (2018) Fucoidan upregulates TLR4/CHOP-mediated caspase-3 and PARP activation to enhance cisplatin-induced cytotoxicity in human lung cancer cells. Cancer Letters 432, 112-120. (IF 9.7, Oncology 34/241=14.11 %)
Jing-Jy Cheng, Chi-Hsein Chao, Mei-Kuang Lu * (2018) Large-scale preparation of sulfated polysaccharides with anti-angionenic and anti-inflammatory properties from Antrodia cinnamomea. International Journal of Biological Macromolecules 113, 1198-1205. (IF 8.2, Polymer Science 5/86=5.81%)
Chia-Chuan Chang *, Jing-Jy Cheng, I-Jung Lee, Mei-Kuang Lu * (2018) Purification, structural elucidation, and anti-inflammatory activity of xylosyl galactofucan from Armillaria mellea. International Journal of Biological Macromolecules 114, 584-591. (IF 8.2, Polymer Science 5/86=5.81%)
Mei-Kuang Lu＊, §, Tung-Yi Lin §, Chun-Hao Hu, Chi-Hsein Chao, Chia-Chuan Chang *, and Hsien-Yeh Hsu＊(2017) Characterization of a sulfated galactoglucan from Antrodia cinnamomea and its anticancer mechanism via TGFβ/FAK/Slug axis suppression. Carbohydrate Polymers 167, 229-239. (IF 11.2, Polymer Science 3/86=3.49%)
Mei-Kuang Lu *, Tung-Yi Lin, Chi-Hsein Chao, Chun-Hao Hu, and Hsien-Yeh Hsu * (2017) Molecular mechanism of Antrodia cinnamomea sulfated polysaccharide on the suppression of lung cancer cell growth and migration via induction of transforming growth factor β receptor degradation. International Journal of Biological Macromolecules 95, 1144-1152. (IF 8.2, Polymer Science 5/86=5.81%)
Hsien-Yeh Hsu *, Tung-Yi Lin *, Mei-Kuang Lu, Pei-Ju Leng, Shu-Ming Tsao1 & Yu-Chung Wu (2017) Fucoidan induces Toll-like receptor 4-regulated reactive oxygen species and promotes endoplasmic reticulum stress-mediated apoptosis in lung cancer. Sci Rep. 7:44990 (IF 4.6, Multidisciplinary Sciences 22/73=30.14)
Yun-Ting Chen, Yuh-Chiang Shen, Meng-Chi Chang, Mei-Kuang Lu * (2016) Precursor-feeding strategy on the triterpenoid production and anti-inflammatory activity of Antrodia cinnamomea. Process Biochemistry 51, 941-949. (IF 4.4, Engineering, Chemical 42/140=30.0%)
Jing-Jy Cheng, Chi-Hsein Chao, Pin-Chun Chang, Mei-Kuang Lu * (2016) Studies on anti-inflammatory activity of sulfated polysaccharides from cultivated fungi Antrodia cinnamomea. Food Hydrocolloids 53, 37-45. (IF 10.7, Food Science & Technology 5/142=3.52 %)
Feng-Lin Hsu, Hsiao-Chuan Chen, and Mei-Kuang Lu * (2016) Polysaccharides from Cunninghamia konishii promote the mycelial growth of Antrodia cinnamomea. Food Hydrocolloids 53, 2-6. (IF 10.7, Food Science & Technology 5/142=3.52 %)
Ching-Wen Chang, Huu-Sheng Lur, Mei-Kuang Lu *, Jing-Jy Cheng * (2013) Sulfated polysaccharides of Armillariella mellea and their anti-inflammatory activities via NF-κB suppression. Food Research International 54, 239-245. (IF 8.1, Food Science & Technology 10/142=7.04%)
Meng-Hsin Lee, Chi-Hsein Chao, Mei-Kuang Lu * (2012) Effect of carbohydrate-based media on the biomass, polysaccharides molecular weight distribution and sugar composition from Pycnoporus sanguineus. Biomass and Bioenergy 47, 37-43. (IF 6.0, Agricultural Engineering 2/14=14.29%)
Jing-Jy Cheng, Chia-Chuan Chang, Chi-Hsein Chao, Mei-Kuang Lu * (2012) Characterization of fungal sulfated polysaccharides and their synergistic anticancer effects with doxorubicin. Carbohydrate Polymers 90, 134-139. (IF 11.2, Polymer Science 3/86=3.49%)
Chung-Io Kuo, Chi-Hsein Chao, Mei-Kuang Lu * (2012) Effects of auxins on the production of steroidal alkaloids in rapidly proliferating tissue and cell cultures of Solanum lyratum. Phytochemical Analysis 23, 400-404. (IF 3.3, Plant Sci 74/238=31.09%)
Chi-Hsein Chao, Mei-Kuang Lu * (2012) Effects of carbon-source on the structural changes of polysaccharides in the dietary mushroom Agaricus subrufescens. Journal of Chinese Medicine 23, 31-40.
Chi-Hsein Chao, Hui-Ju Wu, Mei-Kuang Lu * (2011) Promotion of fungal growth and underlying physiochemical changes of polysaccharides in Rigidoporus ulmarius, an edible Basidiomycete mushroom. Carbohydrate Polymers 85, 609-614. (IF 11.2, Polymer Science 3/86=3.49%)
Tun-Tschu Chang, Chi-Hsein Chao, Mei-Kuang Lu * (2011) Enhanced biomass production of Pycnoporus sanguineus and alterations in the physiochemical properties of its polysaccharides. Carbohydrate Polymers 83, 796-801. (IF 11.2, Polymer Science 3/86=3.49%)
Jing-Jy Cheng 1, Mei-Kuang Lu 1, Cha-Yui Lin, Chia-Chuan Chang * (2011) Characterization and functional elucidation of a fucosylated 1,6-α-d-mannogalactan polysaccharide from Antrodia cinnamomea. Carbohydrate Polymers 83, 545-553. (IF 11.2, Polymer Science 3/86=3.49%) 1 Equally contribute
Men-Chi Chang, Wei-Fu Chien, Chi-Hsein Chao, Mei-Kuang Lu * (2010) Effects of cold stress on alterations of physiochemical and chemical properties of rice polysaccharides. Carbohydrate Polymers 80, 374-377. (IF 11.2, Polymer Science 3/86=3.49%)
Mei-Kuang Lu 1, Jing-Jy Cheng 1, Cha-Yui Lin, Chia-Chuan Chang * (2010) Purification, structural elucidation, and anti-inflammatory effect of a water-soluble 1,6-branched 1,3-α-d-galactan from cultured mycelia of Poria cocos. Food Chemistry 118, 349-356. (IF 8.8, Nutrition & Diet 5/88= 5.68%) 1 Equally contribute
Jing-Jy Cheng 1, Huuheng Lur 1, Nai-Kuei Huang, Hsuan-Pei Chen, Cha-Yui Lin, Mei-Kuang Lu * (2009) Exploring the potential of biopharmaceutical production by Rigidoporus ulmarius: cultivation, chemistry, and bioactivity studies. Process Biochemistry 44, 1237-1244. (IF 4.4, Engineering, Chemical 42/140=30.0%)
Jing-Jy Cheng, Nai-Kuei Huang, Huu-Sheng Lur, Chung-Io Kuo, Mei-Kuang Lu* (2009) Characterization and biological functions of sulfated polysaccharides from sulfated-salt treatment of Antrodia cinnamomea. Process Biochemistry 44, 453-459. (IF 4.4, Engineering, Chemical 42/140=30.0%)
Jing-Jy Cheng, Cha-Yui Lin, Huu-Sheng Lur, Hsuan-Pei Chen, Mei-Kuang Lu* (2008) Properties and biological functions of polysaccharides and ethanolic extracts isolated from medicinal fungus, Fomitopsis pinicola. Process Biochemistry 43, 829-834. (IF 4.4, Engineering, Chemical 42/140=30.0%)
Mei-Kuang Lu, Jing-Jy Cheng, Wen-Lin Lai, Yen-Ru Lin, Nai-Kuei Huang (2008) Fermented Antrodia cinnamomea † extract prevents rat PC12 cells from serum deprivation-induced apoptosis: the role of the MAPK family. Journal of Agricultural Food Chemistry 56, 865-874. (IF 6.1, Agriculture, Multidiscipline 6/58=10.34%)
Meng-Hsin Lee, Jing-Jy Cheng, Cha-Yui Lin, Yi-Jyun Chen, Mei-Kuang Lu* (2007) Precursor feeding strategy for the production of solanine, solanidine and solasodine by a cell culture of Solanum lyratum. Process Biochemistry 42, 899-903. (IF 4.4, Engineering, Chemical 42/140=30.0%)
Feng-Lin Hsu, Cheng-Jen Chou, Yi-Chieh Chang, Tun-Tschu Chang, Mei-Kuang Lu * (2006) Promotion of hyphal growth and underlying chemical changes in Antrodia cinnamomea by host factors from Cinnamomum cinnamomea. International Journal of Food Microbiology 106, 32-38. (IF 5.4 Food Science & Technology 31/142=21.83%)
Mei-Kuang Lu, Jing-Jy Cheng, Wen-Lin Lai, Yen-Ru, Lin, and Nai-Kuei Huang (2006) Adenosine as an active component of Antrodia cinnamomea that prevents rat PC12 cells from serum deprivation-induced apoptosis through the activation of Adenosine A2A receptors. Life Sciences 79, 252-258. (IF 6.1, Pharm & Pharmcol 35/277=12.64%)
Shih Chung Chen1, Mei-Kuang Lu1, Jing-Jy Cheng , Danny Ling Wang (2005) Antiangiogenic activities of polysaccharides isolated from medicinal fungi. FEMS Microbiology Letters 249, 247-254. 1 Equally contribute (IF 2.1, Microbiology 118/135=87.41%)
Jing-Jy Cheng, Chao-Jan Yang, Chia-Hua Cheng, Ya-Ting Wang, Nai-Kuei Huang, Mei- Kuang Lu * (2005) Characterization and functional study of Antrodia camphorata lipopolysaccharide. Journal of Agricultural Food Chemistry 56, 865-874. (IF 6.1, Agriculture, Multidiscipline 6/58=10.34%)
Jing-Jy Cheng, Nai-Kuei Huang, Tun-Tschu Chang, Danny Ling Wang, Mei-Kuang Lu* (2005) Study for anti-angiogenic activities of polysaccharides isolated from Antrodia cinnamomea in endothelial cells. Life Sciences 76, 3029-3042. (IF 6.1, Pharm & Pharmcol 35/277=12.64%)
Nai-Kuei Huang, Jing-Jy Cheng, Wen-Lin Lai, Mei-Kuang Lu* (2005) Antrodia camphorata prevents rat pheochromocytoma cells from serum deprivation-induced apoptosis. FEMS Microbiology Letters 244, 213-219. (IF 2.1, Microbiology 118/135=87.41%)
Yuh-Chiang Shen, Cheng-Jen Chou, Yea-Hwey Wang, Chieh-Fu Chen, Yueh-Chinh Chou, Mei-Kuang Lu* (2004) Anti-inflammatory activity of the extracts from mycelia of Antrodia camphorata cultured with water-soluble fraction from five different Cinnamomum genera. FEMS Microbiology Letters 231, 137-143. (IF 2.1, Microbiology 118/135=87.41%)
Guei-Jane Wang , Hsiang-Wen Tseng , Cheng-Jen Chou , Tung-Hu Tsai , Chi-Tin Chen , Mei-Kuang Lu* (2003) The vasorelaxation of Antrodia camphorata mycelia: involvement of endothelial Ca2+-NO-cGMP pathway. Life Sciences 73, 2769-2783. (IF 6.1, Pharm & Pharmcol 35/277=12.64%)
I-Hung Lee, Ray-Ling Huang, Chi-Ting Chen,Hsiao-Chuan Chen, Wen-Chi Hsu, Mei-Kuang Lu*(2002) Antrodia camphorata polysaccharide exhibits anti-hepatitis B virus effects. FEMS Microbiology Letters 209, 63-67. (IF 2.1, Microbiology 118/135=87.41%)
*Corresponding author
研究計畫-(五年內計畫)
國科會計畫：
1. 提升茯苓硫化多醣生產、硫含量及生物活性的優化培養條件。2023.08.01-2026.07.31。主持人
2. 牛樟芝硫化多醣體的生產，化學輪廓分析，片段化及抗發炎、抗癌活性研究。2022.08.01-2025.07.31。主持人
3. 以質量控制生產及片段化具抗癌、抑制血管新生及抗發炎的牛樟芝硫化多醣體。2019.08.01-2022.07.31。主持人
4. 大量生產優質及片段化具抗癌、抑制血管新生及抗發炎的牛樟芝硫化多醣體。
2016.08.01-2019.07.31。主持人
I have been interested in polysaccharides production, structure and biological function in fungi. Much of the work I have done in the past used Antrodia cinnamomea as a model for polysaccharide structure and function. For the past five years Dr. Tung-Yi Lin and myself have been working on production, purification, and characterization of sulfated polysaccharides in fungi and how this sulfated polysaccharides responds to anti-cancer and anti-inflammatory activities. My interests in this system is how to use cultured mycelia to provide the opportunity to fine-tune the biosynthetic pathway of sulfated polysaccharide. These polysaccharides from different sources could differ somewhat in structure and composition. Different physicochemical parameters of polysaccharides, such as solubility, primary structure, molecular weight, extent of branching by side-chain substituents, and the charge on the polymer, all appear to influence their biological activities.
Recently, we have also created a series of polysaccharides isolated from Poria cocos, Ganoderma lucidum, Pycnoporus sanguineus, and Rigidoporus ulmarius cultured with different carbon-sourced medium. We have examined the physiological consequence of these cultured mycelia. Sulfated polysaccharide was fully characterized according to its molecular weight distribution and sugar composition. We have observed that different carbon sources generate different polymers with different degrees of polymerization, producing more or less water-soluble may possess higher or lower biological activity. We are in the process of bio-activity assay.
In preparation the bioactive sulfated polysaccharide (SPS) of Antrodia cinnamomea we discovered that a sulfated salt (SO4 2-) could change in the sugar compositions, and degree of sulfation in the SPS. The more the SO4 2-, the higher the sulfation degree. We observed that the degree of sulfation affected the antiangiogenic and neuroprotective properties. These results are consistent with those of Parish et al. who found that the inhibitory activity against human angiogenesis of SPS was critically dependent on the chain length and degree of sulfation. We are continuing to characterize the minimum length of sulfated oligosaccharides with the greatest anti-angiogenic activity by using fragmentation of SPS. In addition, we first demonstrated the existence of lipopolysaccharides (LPS) in medicinal fungus, Antrodia cinnamomea (J. Agri. Food Chem. 2005, 53, 469-474). Chemical and functional properties were investigated on the fungus LPS. Compositional analysis revealed that sorbitol, fucose, galactose and glucose were the neutral sugars in LPS of A. cinnamomea. Compared that with Escherichia coli O129 LPS, galactosamine, glucosamine, galactose, and glucose, were the predominant monosaccharide species in E. coli O129 LPS molecules, whereas galactosamine and glucosamine were absent in A. cinnamomea LPS. Since these properties are different from those of bacterial LPS, the function between fungus and bacterial LPS are also discussed.
We also have Dr. Chia-Chuan Chang to join to elucidate partial structural of fungal polysaccharide by using 1H, 13C and 2D NMR spectroscopy, including NOESY and HMBC experiments for linkage and sequence analysis. We are planning to fractionate and identify the structure of active polysaccharides in anti-cancer and anti-inflammation. Recently, we discovered a novel 2-O sulfated 1,3-/1,4-galactoglucan from cultured mycelia of A. cinnamomea (International Journal of Biological Macromolecules 2023, 241, 124559). By comparing the result of cultured mycelia in the same species but grows in the wield, we may uncover novel function of this species. This work has applied implications for the development of candidates for cancer therapy. Submerged culture may increase the yield potential to achieve the demands of commercial-scale of mycelial and polysaccharide production.
In the past 20 years, major research progress focused on (1) In vitro manipulation of the production of Antrodia cinnamomea and its biological activity. (2) Production of medicinal fungus, Poria cocos, Pycnoporus sanguineus, Armillaria mellea, Fomitopsis pinicola, Rigidoporus ulmarius with biological activities. (3) Precursor-feeding strategy to produce effective compounds of plant. These topics and publications are described as follows.
1.In vitro manipulation of the production of Antrodia cinnamomea and its biological activity
We first perform a hybrid machine learning approach (ANFIS-NM) to identify the potent factors and optimize the cultivation conditions of A. cinnamomea (Bioresource Technology 2023, 369, 128412; Biomass and Bioenergy 2022, 158, 106349). We investigated the hyphal growth-promoting factors of Antrodia cinnamomea from the host-related species, Cinnamomum camphora (International Journal of Food Microbiology 2006, 106, 32-38). We identified the HGF was in the polysaccharide fraction of C. camphora, and it maximally stimulated growth compared to that of the control.
We demonstrate the biological functions and the underlying mechanisms of sulfated polysaccharides of A. cinnamomea (International Journal of Biological Macromolecules 2023, 241, 124559; International Journal of Biological Macromolecules 2023, 238, 124144; International Journal of Biological Macromolecules 2023, 226, 1236-1247; International Journal of Biological Macromolecules 2021, 170, 307-316; International Journal of Biological Macromolecules 2020, 162, 1476-1483; International Journal of Biological Macromolecules 2020, 159, 1013-1021; International Journal of Biological Macromolecules 2020, 148, 715-721; Carbohydrate Polymers 2019, 216, 204-212; Carbohydrate Polymers 2019, 210, 175-184; Carbohydrate Polymers 2018, 202, 536-544; International Journal of Biological Macromolecules 2018, 120, 952-958; International Journal of Biological Macromolecules 2018, 113, 1198-1205; Carbohydrate Polymers 2017, 167, 229-239; International Journal of Biological Macromolecules 2017, 95, 1144-1152; Food Hydrocolloids 2016, 53, 37-45; Carbohydrate Polymers 2012, 90, 134-139; Carbohydrate Polymers 2011, 83, 545-553; Process Biochemistry 2009, 44, 453-459). We were the first attempt to purify SPS from a fungal species, A. cinnamomea. We determined the physiological nature and chemical architecture of the bioactive domains of the SPS. We prepared series of SPS in our fermentation library of A. cinnamomea. There were significant qualitative changes in the sugar compositions, and degree of sulfation, which influenced their biological activities.
In addition to sulfated polysaccharide, Dr. Nai-Kuei Huang and myself examined the ethanolic extract of A. cinnamomea both in chemical composition and neuroprotection activity. Dr. Huang used a serum deprivation-induced apoptosis in neuronal-like pheochromocytoma (PC12) cells as a cell stress model, and we found that A. cinnamomea was effective in preventing serum-deprived apoptosis according to results of the MTT assay and Hoechst staining. We demonstrated the active component of A. cinnamomea is adenosine (ADO) and the targeting site in this model is A2A-R(Life Sciences 2006, 79, 225-232). A. cinnamomea prevented serum deprivation-induced PC12 cell apoptosis through a PKA-dependent pathway and suppression of JNK and p38 activities (Journal of Agricultural Food Chemistry 2008, 56, 865-74).
2.Production of medicinal fungus, Poria cocos, Pycnoporus sanguineus, Armillaria mellea, Fomitopsis pinicola, Rigidoporus ulmarius with biological activities
Fomitopsis pinicola is another fungus I am interested. It is now being marketed as a tea and food supplement. The fungus is very common on dead trees and plays a very important ecological role in the degradation of woody forest litter. Although the mechanisms underlying the antitumor effects of the extract have still not been clarified, its tumoricidal effects have been confirmed. We found the highest mycelial yield and polysaccharide production change with the time of cultivation. Harvest time selection is also an important factor to obtain maximal fungal production (Process Biochemistry 2008, 43, 829-834). Both polysaccharides and its ethanolic extract were prepared and showed no toxicity to endothelial cells (ECs). Extracted polysaccharides had a strong inhibitory effect on vascular endothelial growth factor (VEGF)-induced tube formation in ECs in a dose-dependent manner. Furthermore, the ethanolic extract dose-dependently suppressed production of the interferon (IFN)-γ-induced inflammation marker, IP-10. These results suggest that different extracts from F. pinicola play different roles in regulating the angiogenic process and inflammation.
3. Precursor-feeding strategy to produce effective compounds of plant
I am also working on in vitro production of secondary metabolites in plant cell system. I used precursor-feeding strategy on undifferentiated cell cultures of Solanum lyratum, a medicinal plant, to produceα-solanine, solanidine, and solasodine (Process Biochemistry 2007, 42, 899-903). In this study, S. lyratum cells were fed exogenous plant sterols including cholesterol, stigmasterol, and mixed sterols (β-sitosterol, campesterol, and dihydrobrassicasterol). The maximal solasodine level in cells was 11.19 mg/g dry weight (DW) after 0.05~1 mg/l stigmasterol feeding, which was about tenfold higher than the control. With regard to solanidine levels, the maximal level in cells was 5.84 mg/g DW after feeding with 20 mg/l cholesterol. It is evident that precursor-feeding strategy described above is applicable at least to steroidal alkaloids accumulation in suspension cultures of S. lyratum and could be useful for in vitro production of solasodine and solanidine.
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